Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Choroidal and macular thickness changes induced by cataract surgery

doi: 10.2147/OPTH.S53989

Figure Lengend Snippet: Retinal and choroidal thickness measurements. Using the calipers provided by the Spectralis® Heidelberg® software, manual retinal macular thickness measurements were performed at seven different locations centered on the fovea, 500 μm apart (top). A mean retinal thickness value was obtained from these measurements. On the same locations used to measure the retinal thickness, manual choroidal measurements were also performed using the calipers, and a mean choroidal thickness was obtained (bottom). Measurements were performed on both horizontal and vertical scans.

Article Snippet: Preoperatively, the patients underwent EDI SD-OCT using the Spectralis® Heidelberg® apparatus (Heidelberg Engineering, Carlsbad, CA, USA).

Techniques: Software

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Choroidal and macular thickness changes induced by cataract surgery

doi: 10.2147/OPTH.S53989

Figure Lengend Snippet: Peripapillary choroidal thickness measurements. Peripapillary SD-OCT scans were performed using the EDI mode ( A ). The automatic detection of the internal limiting membrane and retinal nerve fiber layer were manually changed so that they would correspond to the external border of the retinal pigment epithelium and the external limit of the choroid respectively ( B ). Automatic readings of the choroidal thickness were obtained in the quadrants provided by the Spectralis® Heidelberg® software ( C ). Choroidal thickness values are the numbers in black. The numbers in brackets and the color-coding refer to the normative database for RNFL evaluation of the Spectralis Heidelberg and are therefore irrelevant for the results. Abbreviations: EDI, enhanced depth imaging; SD-OCT, spectral domain optical coherence tomography; RNFL, retinal nerve fiber layer; G, general; N, nasal; NS, nasal superior; TS, temporal superior; T, temporal; TI, temporal inferior; NI, nasal inferior.

Article Snippet: Preoperatively, the patients underwent EDI SD-OCT using the Spectralis® Heidelberg® apparatus (Heidelberg Engineering, Carlsbad, CA, USA).

Techniques: Membrane, Software, Imaging, Tomography

Journal: Molecular Vision

Article Title: Genetic and environmental risk factors for reticular pseudodrusen in the EUGENDA study

doi:

Figure Lengend Snippet: Imaging of reticular pseudodrusen. Example of reticular pseudodrusen (RPD) visible via fundus autofluorescence ( A ), infrared imaging ( B ) and spectral-domain optical coherence tomography (SD-OCT; C ).

Article Snippet: Retinal images, including SD-OCT volume scans registered over NIR images (Spectralis SDOCT, Heidelberg Engineering, Heidelberg, Germany) and stereo color FP (FP, Cologne: Canon UVI fundus camera using 40° field of view; Canon, Tokyo, Japan, and Nijmegen: Topcon TRC 50IX fundus camera using 50° field of view; Topcon, Tokyo, Japan), were collected from each participant.

Techniques: Imaging, Tomography

Journal: Scientific Reports

Article Title: Identification of new families and variants in autosomal dominant macular dystrophy associated with THRB

doi: 10.1038/s41598-025-97768-9

Figure Lengend Snippet: Ophthalmologic findings in patients with pathogenic variants in THRB in our IRD-cohort. ( A ) Patient IV:2 from Family 1: A1, A2, A4 and A5 correspond to Optos pseudocolour fundus autofluorescence images of the right eye (A1, A2) and left eye (A4, A5). A3 and A6 correspond to SD-OCT of the right and left macula, respectively. ( B ) Patient III:4 from Family 2: B1, B2 (right eye) and B4, B5 (left eye) represent her Optos pseudocolour wide-field images, and B3 (right eye) and B6 (left eye) correspond to her SD-OCT Spectralis scans. ( C ) Patient IV:1 from Family 3: SD-OCT Spectralis scans images are shown of the right (C1, C2) and left (C4, C5) eye. C3 and C6 represent SD-OCT scans of both maculae. ( D ) Patient III:7 from Family 3: Optos pseudocolour wide-field images of the right (D1, D2) and left (D4, D5) eye are shown. D3 and D6 correspond to SD-OCT Spectralis scans of the right and left macula, respectively. ( E ) Patient V:3 from Family 3: E1, E2 and E4, E5 correspond to Optos pseudocolour wide-field images of the right (E1, E2) and left (E4, E5) maculae. E3 and E6 are SD-OCT Spectralis scans of the right and left macula, respectively. ( F ) Patient IV:12 from Family 3: Optos pseudocolour wide-field imaging of the right (F1, F2) and left (F4, F5); and SD-OCT Spectralis scans of the right (F3) and left (F6) macula, showing granularity of the ellipsoid zone subfoveally in both eyes. ( G ) Patient III:4 from Family 3: Both images represent SD-OCT scans of the right (G1) and left (G2) maculae. ( H ) Patient IV:7 from Family 3: Cirrus HD OCT images of the right (H1) and left (H2) eyes.

Article Snippet: D3 and D6 correspond to SD-OCT Spectralis scans of the right and left macula, respectively. ( E ) Patient V:3 from Family 3: E1, E2 and E4, E5 correspond to Optos pseudocolour wide-field images of the right (E1, E2) and left (E4, E5) maculae.

Techniques: Imaging

Journal: Molecular Therapy

Article Title: Successful Gene Therapy in the RPGRIP1-deficient Dog: a Large Model of Cone–Rod Dystrophy

doi: 10.1038/mt.2013.232

Figure Lengend Snippet: In vivo assessment of retinal morphology in dogs A2 and A8 at 3, 9, 12, and 24 mpi. (a) Fundus photographs and time-domain optical coherence tomography (OCT) images obtained for the right and left eyes of control nonaffected, untreated dog NA1 at 24 months of age. (b) Fundus photographs and retinal cross-sectional images obtained for the treated and untreated eyes of dog A2 treated with AAV2/5-RK-cRpgrip1 at 3, 9, 12, and 24 months postinjection (mpi). (c) Fundus photographs and retinal cross-sectional images obtained for the treated and untreated eyes of dog A8 treated with AAV2/8-RK-cRpgrip1 at 3, 9, 12, and 24 mpi. White-dotted circles on fundus photographs schematically represent areas of the treated retinas exposed to rAAV vectors. OCT scans were acquired on a horizontal line shown on the fundus images (white lines). The localization and the size of the white lines represent the localization and the size of the OCT scans. Retinal thicknesses at the same location (13 mm above the optic nerve head) were measured using calibrated calipers and are indicated on the OCT scans. m, months of age; T, treated eye; U, untreated eye.

Article Snippet: SD-OCT was performed with 30° horizontal scans placed across the vector bleb temporal boundary (Spectralis Heidelberg Engineering, Heidelberg, Germany).

Techniques: In Vivo, Tomography, Control

Journal: Molecular Therapy

Article Title: Successful Gene Therapy in the RPGRIP1-deficient Dog: a Large Model of Cone–Rod Dystrophy

doi: 10.1038/mt.2013.232

Figure Lengend Snippet: In vivo and postmortem assessment of photoreceptor (PR) layer thickness in dog A2 at 24 months postinjection (mpi). (a) Topographies of PR layer and spectral-domain optical coherence tomography (SD-OCT) scans (obtained 13 mm above the optic nerve head) from the right eye of dog NA1 (top) and both untreated (middle) and treated (bottom) eyes of dog A2 at 24 mpi. White-dotted circles on fundus photographs schematically represent the area of the treated A2 retina exposed to AAV2/5-RK-cRpgrip1 vector. OCT scans were acquired on a horizontal line shown on the fundus images (white lines). PR layer (outer nuclear layer (ONL) + outer segments (OSs) and inner segments (ISs)) was defined semi-automatically (red). PR layer thickness was measured in two normalized (1) temporal and (2) nasal locations. (b,c) Microscopic images of retinal sections from the treated eye of dog A2 at 24 mpi and the right eye of age-matched control dog NA1. Serial retinal cryosections encompassing the vector-exposed and the vector-unexposed areas of the treated retina were (b) stained with hematoxylin and eosin (H&E) (top) or (c) immunolabeled against GNAT1 (red, middle) or peanut agglutinin-FITC (green, bottom). Primary GNAT1 antibody was detected with Alexa 546–conjugated goat antirabbit IgG. Scale bar = 30 µm. GCL, ganglion cell layer; INL, inner nuclear layer; m, months of age; RPE, retinal pigment epithelium.

Article Snippet: SD-OCT was performed with 30° horizontal scans placed across the vector bleb temporal boundary (Spectralis Heidelberg Engineering, Heidelberg, Germany).

Techniques: In Vivo, Tomography, Plasmid Preparation, Control, Staining, Immunolabeling